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Did Jesus have fleshly brothers and sisters? Yes, we know that he did, because Matthew 12:46-50 make it very clear.
There we read that while he was indoors speaking, his mother (Mary) and his brothers (James, JoSeph, Simon, and Judas) had been standing outside waiting to talk to him. Further, at Acts 1:14, we read that Jesus’ mother and brothers were present (along with his Apostles) shortly after he ascended to heaven.
However, were these really Jesus’ fleshly brothers, or could they just be ‘spiritual’ brothers?
The context provides the answer. Since Jesus’ brothers are mentioned as being there along with his Apostles, his disciples, and his mother (Mary) in the account in Acts, by elimination, these had to be his fleshly (half) brothers, that is, other children of Mary.